| Title | Differentiation of Salmonella typhimurium from Gram-negative Intestinal Microbes by Randomly Amplified Polymorphic DNA (RAPD) Fingerprinting |
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| Author | Un-Ho Jin, Tae-Wook Chung, June-Ki Kim, Kyung-Soo Nam 1 , Sang-Do Ha 2 , and Cheorl-Ho Kim * |
| Address | Department of Biochemistry, Molecular Biology and Pathology, College of Oriental Medicine, 1 Department of Pharmacology, College of Medicine, Dongguk |
| Bibliography | Journal of Microbiology, 38(1),8-10, 2000, |
| DOI | |
| Key Words | Randomly amplified polymorphic DNA (RAPD), fingerprinting, Salmonella typhimurium |
| Abstract | In order to rapidly identify and differentiate Salmonella typhimurium from the intestinal gram-negative bacteria, randomly amplified polymorphic DNA (RAPD) fingerprinting of Salmonella typhimurium was carried out using random primers designated OPA-13 (5'-CAGCACCCAC-3'), OPB-10 (5'-CGT-CTGGGAC-3'), OPB-18 (5'-CCACAGCAGT-3'), and OPJ-10 (5'-AAGCCCGAGG-3'), and its patterns compared with 6 representive intestinal, gram-negative bacterial strains, Vibrio parahaemolyticus, V. vulnificus, Enterobacter cloacae, Escherichia coli O157:H7, Pseudomonas aeruginosa, and Proteus sp., which are often found in foods. S. typhimurium had unique and distinct fingerprinting patterns. RAPD fingerprinting is thus concluded to be a rapid and sensitive method for the identification of S. typh-imurium compared to conventional culturing procedures or immunoassays. |
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