| Title | Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium |
|---|---|
| Author | So Young Yoo1, Pyung Sik Kim2, Ho-Keel Hwang2, Seong-Hoon Lim3, Kwang-Won Kim3, Son-Jin Choe4, Byung-Moo Min5, and Joong-Ki Kook1,* |
| Address | 1Department of Oral Biochemistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea , 2Department of Conservative Dentistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea, 3Department of Orthodontics, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea, 4Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea , 5Department of Oral Biochemistry, College of Dentistry, Seoul National University, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea |
| Bibliography | Journal of Microbiology, 43(2),204-208, 2005, |
| DOI | |
| Key Words | dental plaque, mitis-salivarius bacitracin agar, non-mutans streptococci organism, 16S rDNA |
| Abstract | The objective of this study was to both isolate and identify non-mutans streptococci organisms (non-MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on MSB. The bacteria growing on the MSB plates were then identified with biochemical tests, as well as with 16S rDNA cloning and sequencing techniques. Our data indicated that bacteria from 30 subjects had been recovered on the MSB plates. Among the 21 typical colonies selected from the 30 subjects, 12 colonies, derived from 10 subjects, were identified as non-MSO. These 12 colonies were determined to be Streptococcus anginosus (8 colonies), S. sanguinis (1 colony), and Pantoea agglomerans (3 colonies). These results strongly suggest that a new selective medium will be required for the reliable isolation of mutans streptococci. |
| Download PDF | p.204-208.pdf |