| Title | Functional Characterization of the Gene Encoding UDP-glucose: Tetrahydrobiopterin [alpha]-Glucosyltransferase in Synechococcus sp. PCC 7942 |
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| Author | En-Young Cha1, Jeong Soon Park1, Sireong Jeon1, Jin Seon Kong1, Yong Kee Choi1, Jee-Youn Ryu2, Youn-Il Park2, and Young Shik Park1,* |
| Address | 1School of Biotechnology and Biomedical Science, Inje University, Kimhae 621-749, Republic of Korea, 2Department of Biology, Chungnam National University, Daejon 305-764, Republic of Korea |
| Bibliography | Journal of Microbiology, 43(2),191-195, 2005, |
| DOI | |
| Key Words | tetrahydrobiopterin-glucoside, proteomics, phycocyanin rod linker, photosynthesis, photoprotection, cyanobacteria |
| Abstract | In this study, we attempted to characterize the Synechococcus sp. PCC 7942 mutant resultant from a disruption in the gene encoding UDP-glucose: tetrahydrobiopterin [alpha]-glucosyltransferase (BGluT). 2D-PAGE followed by MALDI-TOF mass spectrometry revealed that phycocyanin rod linker protein 33K was one of the proteins expressed at lower level in the BGluT mutant. BGluT mutant cells were also determined to be more sensitive to high light stress. This is because photosynthetic O_2 exchange rates were significantly decreased, due to the reduced number of functional PSIs relative to the wild type cells. These results suggested that, in Synechococcus sp. PCC 7942, BH4-glucoside might be involved in photosynthetic photoprotection. |
| Download PDF | p.191-195.pdf |