| Title | Enhancement of Gene Delivery to Cancer Cells by a Retargeted Adenovirus |
|---|---|
| Author | Kwang Seok Oh1, Jeffrey A. Engler2, and Insil Joung1 |
| Address | 1Department of Biology, Hanseo University, Seosan, Chungnam 356-706, Republic of Korea, 2Departments of Biochemistry and Molecular Genetics, Schools of Medicine and Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294-0005, USA |
| Bibliography | Journal of Microbiology, 43(2),179-182, 2005, |
| DOI | |
| Key Words | Adenovirus, cancer cell, ligand-modified fiber, targeting |
| Abstract | The inefficiency of in vivo gene transfer using currently available vectors reflects a major hurdle in cancer gene therapy. Both viral and non-viral approaches that improve gene transfer efficiency have been described, but suffer from a number of limitations. Herein, a fiber-modified adenovirus, carrying the small peptide ligand on the capsid, was tested for the delivery of a transgene to cancer cells. The fiber-modified adenovirus was able to mediate the entry and expression of a [beta]-galactosidase into cancer cells with increased efficiency compared to the unmodified adenovirus. Particularly, the gene transfer efficiency was improved up to 5 times in OVCAR3 cells, an ovarian cancer cell line. Such transduction systems hold promise for delivering genes to transferrin receptor overexpressing cancer cells, and could be used for future cancer gene therapy. |
| Download PDF | p.179-182.pdf |