| Title | Sphingopyxis granuli sp. nov., a [beta]-Glucosidase-Producing Bacterium in the Family Sphingomonadaceae in [alpha]-4 Subclass of the Proteobacteria |
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| Author | Myung Kyum Kim1, Wan-Taek Im1, Hiroyuki Ohta2, Myungjin Lee1,3, and Sung-Taik Lee1,* |
| Address | 1Environmental and Molecular Microbiology Lab., Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, Republic of Korea, 2Department of Bioresource Science, Ibaraki University College of Agriculture, Ami-machi, Ibaraki 300-0393, Japan, 3NeoPharm Co., Ltd., BVC-307, KRIBB, 52 Oeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea |
| Bibliography | Journal of Microbiology, 43(2),152-157, 2005, |
| DOI | |
| Key Words | [alpha]-Proteobacteria, Sphingomonadaceae, Sphingopyxis granuli |
| Abstract | Strain Kw07^T, a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from granules in an Up-flow Anaerobic Sludge Blanket (UASB) bioreactor used in the treatment of brewery wastewater. 16S rRNA gene sequence analysis revealed that strain Kw07^T belongs to the [alpha]-4 subclass of the Proteobacteria, and the highest degree of sequence similarity was determined to be to Sphingopyxis macrogoltabida IFO 15033^T (97.8%). Chemotaxonomic data revealed that strain Kw07^T possesses a quinone system with the predominant compound Q-10, the predominant fatty acid C_18:1 w7c, and sphingolipids, all of which corroborated our assignment of the strain to the Sphingopyxis genus. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Kw07^T represents a distinct species. Based on these data, Kw07^T (= KCTC 12209^T = NBRC 100800^T) should be classified as the type strain for a novel Sphingopyxis species, for which the name Sphingopyxis granuli sp. nov. has been proposed. |
| Download PDF | p.152-157.pdf |