| Title | Production of Saccharogenic and Dextrinogenic Amylases by Rhizomucor pusillus A 13.36 |
|---|---|
| Author | Tony M. Silva1, Derlene Attili-Angelis2, Ana Flavia Azevedo Carvalho1, Roberto Da Silva1, Mauricio Boscolo3, and Eleni Gomes1,* |
| Address | 1Laboratorio de Bioquimica e Microbiologia, IBILCE-Instituto de Instituto de Biociencias, Letras e Ciencias Exatas, UNESP- Universidade Estadual Paulista., Sao Jose do Rio Preto- SP, Brazil, 2Departamento de Bioquimica e Microbiologia, Instituto de Biociencias, UNESP-Universidade EstadualPaulista, Rio Claro-SP, Brazil, 3Laboratorio de Fisico-Quimica, IBILCE-Instituto de Instituto de Biociencias, Letras e Ciencias Exatas,UNESP-Universidade Estadual Paulista., Sao Jose do Rio Preto- SP, Brazil |
| Bibliography | Journal of Microbiology, 43(6),561-568, 2005, |
| DOI | |
| Key Words | amylase, Rhizomucor pusillus, thermostability |
| Abstract | A newly-isolated thermophilic strain of the zygomycete fungus Rhizomucor pusillus 13.36 produced highly active dextrinogenic and saccharogenic enzymes. Cassava pulp was a good alternative substrate for amylase production. Dextrinogenic and saccharogenic amylases exhibited optimum activities at a pH of 4.0-4.5 and 5.0 respectively and at a temperature of 75oC. The enzymes were highly thermostable, with no detectable loss of saccharogenic or dextrinogenic activity after 1 h and 6 h at 60oC, respectively. The saccharogenic activity was inhibited by Ca2+ while the dextrinogenic was indifferent to this ion. Both activities were inhibited by Fe2+ and Cu2+ Hydrolysis of soluble starch by the crude enzyme yielded 66% glucose, 19.5% maltose, 7.7% maltotriose and 6.6% oligosaccharides. |
| Download PDF | 561-568.pdf |