| Title | Screening of Growth- or Development-related Genes by Using Genomic Library with Inducible Promoter in Aspergillus nidulans |
|---|---|
| Author | Bang-Yong Lee1, Sang-Yong Han1, Han Gil Choi1, Jee Hyun Kim2, Kap-Hoon Han3, and Dong-Min Han2,* |
| Address | 1Department of Biology, Graduate School of Wonkwang University, Iksan, 570-749, Republic of Korea, 2Institute of Basic Natural Science, Wonkwang University, Iksan, 570-749, Republic of Korea, 3Research Center for Biomedicinal Resources, Pai Chai University, Daejeon, 302-735, Republic of Korea |
| Bibliography | Journal of Microbiology, 43(6),523-528, 2005, |
| DOI | |
| Key Words | Aspergillus nidulans, NOT mutants, over-expression library, sexual development |
| Abstract | Using the genomic library constructed at the downstream of the niiA promoter, which induces the over-expression of an inserted DNA fragment, we have attempted to screen the genes affecting growth or development by over-expression. The wild-type strain was transformed using the AMA-niiA(p) library and cultured on 1.2 M sorbitol media, in which asexual sporulation is induced, but sexual development is repressed. Over 100,000 strains transformed to pyrG+ were analyzed with regard to any changes in phenotype. Consequently, seven strains were isolated for further analyses. These strains were designated NOT [niiA(p) over-expression transformants] stains. Four of the strains were of the inducible type, and the remaining strains were of the multi-copy suppression type. Two of the inducible-type strains, NOT1 and NOT40, harbored genes which had been inserted in reverse direction, suggesting that the mutant phenotypes had been derived from an excess amount of anti-sense mRNA. Domain analyses of the deduced polypeptides from the DNA fragments rescued from the transformants revealed that NOT1, NOT40 and NOT6 harbored a LisH motif, a forkhead domain, and a Zn(II)2Cys6 binuclear zinc cluster, respectively. |
| Download PDF | 523-528.pdf |