| Title | Carbon Source-Dependent Regulation of the Schizosaccharomyces pombe pbh1 Gene |
|---|---|
| Author | Su-Jung Kim1, Nam-Chul Cho1, In Wang Ryu1, Kyunghoon Kim1, Eun-Hee Park2, and Chang-Jin Lim1* |
| Address | 1Division of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2College of Pharmacy, Sookmyung Women’s University, Seoul 140-742, Republic of Korea |
| Bibliography | Journal of Microbiology, 44(6),689-693, 2006, |
| DOI | |
| Key Words | carbon source, fission yeast, Pap1, Pbh1, Schizosaccharomyces pombe, transcriptional regulation |
| Abstract | Pbh1, from the fission yeast Schizosaccharomyces pombe, is a baculoviral inhibitor of apoptosis (IAP) repeat (BIR) domain-containing protein. Its unique encoding gene was previously found to be regulated by nitric oxide and nitrogen starvation. In the current work, the Pbh1-lacZ fusion gene was used to elucidate the transcriptional regulation of the pbh1 gene under various carbon sources. When fermentable carbon sources, such as glucose (at a low concentration of 0.2%), sucrose (2.0%) and lactose (2.0%), were the sole carbon source, the synthesis of β-galactosidase from the Pbh1-lacZ fusion gene was reasonably enhanced. However, the induction by these fermentable carbon sources was abolished in the Pap1-negative S. pombe cells, implying that this type of induction of the pbh1 gene is mediated by Pap1. Ethanol (2.0%), a nonfermentable carbon source, was also able to enhance the synthesis of β-galactosidase from the fusion gene in wild-type cells but not in Pap1-negative cells. The results indicate that the S. pombe pbh1 gene is up-regulated under metabolic oxidative stress in a Pap1-dependent manner. |
| Download PDF | JM44(6)-17.pdf |