| Title | Cloning and Analysis of a Type II Polyketide Synthase Gene Cluster from Streptomyces toxytricini NRRL 15,443 |
|---|---|
| Author | Anna Yoo1, Atanas V. Demirev2, Ji Seon Lee2, Sang Dal Kim2, and Doo Hyun Nam1* |
| Address | 1Faculty of Pharmacy, Yeungnam University, 214-1 Dae-dong, Kyongsan 712-749, Republic of Korea, 2Faculty of Biotechnology, Yeungnam University, 214-1 Dae-dong, Kyongsan 712-749, Republic of Korea |
| Bibliography | Journal of Microbiology, 44(6),649-654, 2006, |
| DOI | |
| Key Words | Type II polyketide synthase, S. toxytricini, lipstatin, β-ketoacyl synthase, chain length factor, acyl carrier protein, thioesterase |
| Abstract | A standard type II polyketide synthase (PKS) gene cluster was isolated while attempting to clone the biosynthetic gene for lipstatin from Streptomyces toxytricini NRRL 15,443. This result was observed using a Southern blot of a PstI-digested S. toxytricini chromosomal DNA library with a 444 bp amplified probe of a ketosynthase (KS) gene fragment. Four open reading frames [thioesterase (TE), β-ketoacyl systhase (KAS), chain length factor (CLF), and acyl carrier protein (ACP)], were identified through the nucleotide sequence determination and analysis of a 4.5 kb cloned DNA fragment. In order to confirm the involvement of a cloned gene in lipstatin biosynthesis, a gene disruption experiment for the KS gene was performed. However, the resulting gene disruptant did not show any significant difference in lipstatin production when compared to wild-type S. toxytricini. This result suggests that lipstatin may not be synthesized by a type II PKS. |
| Download PDF | JM44(6)-09.pdf |