| Title | Schizosaccharomyces pombe nup97, which Genetically Interacts with mex67, is Essential for Growth and Involved in mRNA Export |
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| Author | Hyun Jin Cho, Duk Kyung Hwang, Sun Im Jung, and Jin Ho Yoon* |
| Address | Deptartment of Biology, Institute of Basic Sciences, Sungshin Women's University, Seoul 136-742, Republic of Korea |
| Bibliography | Journal of Microbiology, 45(4),344-349, 2007, |
| DOI | |
| Key Words | nup97, mex67, synthetic lethality, mRNA export, Schizosaccharomyces pombe |
| Abstract | We have isolated previously three synthetic lethal mutants in Schizosaccharomyces pombe, which genetically interact with mex67, in order to identify the genes involved in mRNA export. A novel nup97 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex3. The nup97 gene contains one intron and encodes an 851 amino-acid protein that is similar to nucleoporins, Npp106p in S. pombe and Nic96p in Saccharomyces cerevisiae. The nup97 gene is essential for vegetative growth, and nup97 null mutant harboring pREP41X-Nup97 showed poly(A)+ RNA export defect when expression of nup97 is repressed in the presence of thiamine. These results suggest that nup97 is involved in mRNA export from the nucleus to cytoplasm. |
| Download PDF | 45(4)_11.pdf |