| Title | Screening of Genes Related to Methylglyoxal Susceptibility |
|---|---|
| Author | Insook Kim1, Joonho Kim1, Bumchan Min2, Changhan Lee1, and Chankyu Park1* |
| Address | 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea, 2Analytical Science Center, Samyang Central R & D Institute, Daejeon 305-717, Republic of Korea |
| Bibliography | Journal of Microbiology, 45(4),339-343, 2007, |
| DOI | |
| Key Words | genome screening, methylglyoxal, dihydroxyacetone, glyoxalase |
| Abstract | Methylglyoxal (MG) is a reactive metabolite known to accumulate in certain physiological conditions. We attempted to isolate genes associated with this metabolite by genome-wide mutagenesis with TnphoA derivative. After screening on methylglyoxal-containing plate, we obtained insertions in three different genes, ydbD, yjjQ, and yqiI, which gave rise to reproducible MG-sensitive phenotypes in glyoxalase-deficient strain. In addition to its MG sensitivity, the insertion in yqiI exhibited an impaired motility resulting from a reduced flagellar expression. |
| Download PDF | 45(4)_10.pdf |