| Title | Induction of Cytokines and Nitric Oxide in Murine Macrophages Stimulated with Enzymatically Digested Lactobacillus Strains |
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| Author | Dong Woon Kim1*, Sung Back Cho1, Cheol Heui Yun2, Ha Yeon Jeong1, Wan Tae Chung1, Chang Weon Choi1, Hyun Jeong Lee1, In Sik Nam1, Guk Hyun Suh1, Sang Suk Lee3, and Byong Seak Lee1 |
| Address | 1Animal Nutrition and Physiology Division, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea, 2School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea, 3Department of Animal Science and Technology, Sunchon National University, Sunchon 540-742, Republic of Korea |
| Bibliography | Journal of Microbiology, 45(5),373-378, 2007, |
| DOI | |
| Key Words | macrophage, nitric oxide, cytokine, lactobacilli |
| Abstract | Based on observations that lactic acid bacteria have the ability to activate macrophages, we assessed the potential effects of eight different Lactobacillus strains treated with gastrointestinal enzymes on the production of nitric oxide and various cytokines in macrophages. RAW 264.7 murine macrophage cells were cultured with either precipitates or supernatants of Lactobacillus strains digested with pepsin followed by pancreatin. The increased production of nitric oxide and interleukin (IL)-1β, IL-6, IL-12 and tumour necrosis factor (TNF)-α were observed when cultured with precipitates, and this effect was largely strain-dependent. In contrast, the exposure of RAW 264.7 cells to supernatants produced weaker or nearly undetectable effects in comparison to the effects of exposure to precipitates. The induction of nitric oxide appeared to be unaffected. These results demonstrate that nitric oxide and cytokines were effectively induced when the bacterial precipitate was treated with macrophages. The results of the present study also indicate that Lactobacillus strains treated with digestive enzymes are capable of stimulating the production of nitric oxide and cytokines in macrophages, which may modulate the gastrointestinal immune function of the host when it is given as a feed additive. |
| Download PDF | 45(5)_01.pdf |