| Title | Partial Purification of Factors for Differential Transcription of the rrnD Promoters for Ribosomal RNA Synthesis in Streptomyces coelicolor |
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| Author | Mi-Young Hahn1 and Jung-Hye Roe2* |
| Address | 1Genome Research Center for Respiratory Pathogens, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea, 2School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea |
| Bibliography | Journal of Microbiology, 45(6),534-540, 2007, |
| DOI | |
| Key Words | ribosomal RNA, RNA polymerase, transcription, reconstitution |
| Abstract | The Streptomyces coelicolor A3(2) genome contains six operons (rrnA to F) for ribosomal RNA synthesis. Transcription from rrnD occurs from four promoters (p1 to p4). We found that transcripts from the p1 and p3 promoters were most abundant in vivo in the early exponential phase. However, at later phases of exponential and stationary growth, transcripts from the p1 promoter decreased drastically, with the p3 and p4 transcripts constituting the major forms. Partially purified RNA polymerase supported transcription from the p3 and p4 promoters, whereas pure reconstituted RNA polymerase with core enzyme (E) and the major vegetative sigma factor sigmaHrdB (E.sigmaHrdB) did not. In order to assess any potential requirement for additional factor(s) that allow transcription from the p3 and p4 promoters, we fractionated a partially purified RNA polymerase preparation by denaturing gel filtration chromatography. We found that transcription from the p3 and p4 promoters required factor(s) of about 30-35 kDa in addition to RNAP holoenzyme (E.sigmaHrdB). Therefore, transcription from the p3 and p4 promoters, which contain a consensus -10 region but no -35 for sigmaHrdB recognition, are likely to be regulated by transcription factor(s) that modulate RNA polymerase holoenzyme activity in S. coelicolor. |
| Download PDF | 45(6)_10.pdf |