| Title |
The Fission Yeast Homologue of Gle1 is Essential for Growth and Involved in mRNA Export |
| Author |
DongGeRaMi Moon1, Jin-Ah Bae2, Hyun Jin Cho1, and Jin Ho Yoon1* |
| Address |
1Department of Biology, Institute of Basic Sciences, Sungshin Women's University, Seoul 136-742, Republic of Korea , 2Department of Education, Sungshin Women's University, Seoul 136-742, Republic of Korea |
| Bibliography |
Journal of Microbiology, 46(4),422-428, 2008,
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| DOI |
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| Key Words |
mRNA export, Schizosaccharomyces pombe, spgle1 |
| Abstract |
We have isolated Gle1 homologue (named as spgle1) as a partial multicopy suppressor of the synthetic lethality of rae1-167 elf1-21 in fission yeast Schizosaccharomyces pombe. The spgle1 is also able to complement partially temperature-sensitive phenotype of rae1-167 only at a lower restrictive temperature. The spgle1 gene contains one intron and encodes a 480 amino-acid protein with predicted molecular weight of 56.2 kDa. We showed that spgle1 gene is essential for vegetative growth and functional Gle1-GFP protein is localized mainly in NPC. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of spgle1 is repressed or over-expressed. These results suggest that the spGle1 protein is also involved in mRNA export in fission yeast. |
