| Title |
Cloning and Characterization of a Na+/H+ Antiporter Gene of the Moderately Halophilic Bacterium Halobacillus aidingensis AD-6T |
| Author |
Ya Jie Zou1, Li Fu Yang1,2, Lei Wang1, and Su Sheng Yang1* |
| Address |
1Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, and Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, Beijing 100094, P. R. China, 2Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, and Key Laboratory of Physiology for Tropical Crops, Ministry of Agriculture, Danzhou Hainan 571737, P. R. China |
| Bibliography |
Journal of Microbiology, 46(4),415-421, 2008,
|
| DOI |
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| Key Words |
Halobacillus aidingensis, Na+/H+ antiporter, nhaH, Inverse PCR |
| Abstract |
A gene encoding a Na+/H+ antiporter was obtained from the genome of Halobacillus aidingensis AD-6T, which was sequenced and designated as nhaH. The deduced amino acid sequence of the gene was 91% identical to the NhaH of H. dabanensis, and shared 54% identity with the NhaG of Bacillus subtilis. The cloned gene enable the Escherichia coli KNabc cell, which lack all of the major Na+/H+ antiporters, to grow in medium containing 0.2 M NaCl or 10 mM LiCl. The nhaH gene was predicted to encode a 43.5 kDa protein (403 amino acid residues) with 11 putative transmembrane regions. Everted membrane vesicles prepared from E. coli KNabc cells carrying NhaH exhibited Na+/H+ as well as Li+/H+ antiporter activity, which was pH-dependent with the highest activity at pH 8.0, and no K+ /H+ antiporter activity was detected. The deletion of hydrophilic C-terminal amino acid residues showed that the short C-terminal tail was vital for Na+/H+ antiporter activity. |
