| Title |
Protective Effect of Polygoni Cuspidati Radix and Emodin on Vibrio vulnificus Cytotoxicity and Infection |
| Author |
Jong Ro Kim1,2, Dool-Ri Oh1,2, Mi Hye Cha1,2, Byoung Sik Pyo1, Joon Haeng Rhee2,3,4, Hyon E. Choy2,4, Won Keun Oh5, and Young Ran Kim1,2* |
| Address |
1Department of Oriental Medicine Materials, Dongshin University, Jeonnam 520-714, Republic of Korea, 2Clinical Vaccine R&D Center, 3National Research Laboratory of Molecular Microbial Pathogenesis, 4Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea, 5College of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea |
| Bibliography |
Journal of Microbiology, 46(6),737-743, 2008,
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| DOI |
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| Key Words |
V. vulnificus infection, emodin, Polygoni Cuspidati Radix, cytotoxicity, antibacterial activity |
| Abstract |
Vibrio vulnificus, a good model organism of bacterial septicemia, causes fatal septicemia manifesting a fulminating course and a high mortality rate within days. In order to identify new natural substances preventing V. vulnificus infection, a plant library was screened for inhibiting cytotoxicity to host cells by using Trypan blue staining and LDH assay. We found that Polygoni Cuspidati Radix potently suppressed the acute death of HeLa and RAW264.7 cells in a dose dependent manner. Further studies revealed that Polygoni Cuspidati Radix inhibited V. vulnificus growth and survival in HI broth and seawater, respectively. We confirmed that Polygoni Cuspidati Radix contained high level of emodin by thin layer chromatography (TLC). Emodin showed direct antibacterial activity against V. vulnificus. In addition, emodin prevented the morphologic damages and acute death of HeLa cells caused from V. vulnificus. The safety of Polygoni Cuspidati Radix and emodin to host cells was confirmed by MTT assay. Polygoni Cuspidati Radix and emodin protected mice from V. vulnificus infection. |
