| Title |
Molecular Characterization of ORFs 2 to 7 of Korean Porcine Reproductive and Respiratory Syndrome Virus (CA) and Its Protein Expression by Recombinant Baculoviruses |
| Author |
Hyun Na Koo1, Jeong Mi Oh1, Jae Kyung Lee1, Jae Young Choi2,3, Kwang Sik Lee4, Jong Yul Roh2, Yeon Ho Je2, Byung Rae Jin4, Sung Sik Yoo5, Jae Su Kim6, Young In Kim6, In Joong Yoon5, and Soo Dong Woo1* |
| Address |
1Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea, 2School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea, 3Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea, 4College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea, 5Choong-Ang Vaccine Laboratory, Daejeon 305-348, Republic of Korea, 6AgroLife Research Institute (ARI), Dongbu HiTek Co. Ltd., Daejeon 305-708, Republic of Korea |
| Bibliography |
Journal of Microbiology, 46(6),709-719, 2008,
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| DOI |
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| Key Words |
PRRSV, CA strain, phylogenetic analysis, baculovirus, bApGOZA system |
| Abstract |
To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR-2332. Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support. Additionally, expression levels of the PRRSV proteins in insect cells were strong or partially weak. The results of this study have implications for both the taxonomy of PRRSV and vaccine development. |
