| Title |
Molecular and Phylogenetic Characterization of Spodoptera litura Granulovirus |
| Author |
Yong Wang1, Jae Young Choi2, Jong Yul Roh1, Soo Dong Woo3, Byung Rae Jin4, and Yeon Ho Je1* |
| Address |
1Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea, 2Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea, 3College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea, 4College of Natural Resources and Life Science, Dong-A University, Pusan 604-714, Republic of Korea |
| Bibliography |
Journal of Microbiology, 46(6),704-708, 2008,
|
| DOI |
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| Key Words |
baculovirus, Spodoptera litura, granulovirus, SlGV, granulin |
| Abstract |
Baculovirus, Spodoptera litura granulovirus (SlGV) was isolated from the infected S. litura larvae, and was characterized. The granule of SlGV was ovoidal shape with an approximate size of 240~340 nm×140~180 nm. Each granule contained one single rod-shape virion with a mean size of 180~200 nm×20~40 nm. Restriction endonuclease fragment analysis estimated that the total genome size of SlGV is about 115 kb. Necleotide sequence analysis of the granulin gene showed that the gene encodes 249 amino acids with a predicted molecular mass of 29 kDa. When the phylogenic relationship was analyzed using the nucleotide sequence of the granulin gene, SlGV was most closely related to Trichoplusia ni granulovirus (TnGV) and Xestia c-nigrum granulovirus (XcGV) which belong to Type I granulovirus. |
