| Title |
Diversity of Bacterial Community in Freshwater of Woopo Wetland |
| Author |
Keun Sik Baik1, Seong Chan Park1, Eun Mi Kim1,2, Kyung Sook Bae3, Jae-Hyung Ahn4, Jong-Ok Ka4, Jongsik Chun5, and Chi Nam Seong1* |
| Address |
1Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea, 2Department of Dental Hygiene, Gwangju Health College University, Gwangju 506-701, Republic of Korea, 3Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Republic of Korea, 4Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea, 5School of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea |
| Bibliography |
Journal of Microbiology, 46(6),647-655, 2008,
|
| DOI |
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| Key Words |
diversity, Woopo wetland, bacterial isolates, 16S rRNA gene clones, phylotypes |
| Abstract |
Diversity of bacterial community in water layer of Woopo wetland was investigated. Cultivable bacterial strains were isolated by the standard dilution plating technique and culture-independent 16S rRNA gene clones were obtained directly from DNA extracts of a water sample. Amplified rDNA restriction analysis (ARDRA) was applied onto both of the isolates and 16S rRNA gene clones. Rarefaction curves, coverage rate and diversity indices of ARDRA patterns were calculated. Representative isolates and clones of all the single isolate/clone phylotype were partially sequenced and analyzed phylogenetically. Sixty-four and 125 phylotypes were obtained from 203 bacterial isolates and 235 culture-independent 16S rRNA gene clones, respectively. Bacterial isolates were composed of 4 phyla, of which Firmicutes (49.8%) and Actinobacteria (32.0%) were predominant. Isolates were affiliated with 58 species. Culture-independent 16S rRNA gene clones were composed of 8 phyla, of which Proteobacteria (62.2%), Actinobacteria (15.5%), and Bacteroidetes (13.7%) were predominant. Diversity of 16S rRNA gene clones originated from cultivation-independent DNA extracts was higher than that of isolated bacteria. |
