| Title |
Characterization of a Baculovirus Newly Isolated from the Tea Slug Moth, Iragoidae fasciata |
| Author |
Li-Rong Yang1,2, Xiao Qiang3, Bao-Qin Zhang1, Mei-Jun Tang3, and Chuan-Xi Zhang1* |
| Address |
1Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, P. R. China, 2Henan Academy of Agricultural Sciences, Zhengzhou 450002, P. R. China, 3Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, Zhejiang 310000, P. R. China |
| Bibliography |
Journal of Microbiology, 47(2),208-213, 2009,
|
| DOI |
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| Key Words |
Iragoidae fasciata, nucleopolyhedrovirus, SNPV, polyhedrin region, phylogeny, permissive cell line |
| Abstract |
The tea slug moth Iragoidae fasciata (Lepidoptera, Eucleidae) is one of the main insect pests that attack tea bushes. A new nucleopolyhedrovirus (NPV) called Iragoidae fasciata NPV (IrfaNPV) was recently isolated from diseased larvae. An 11,626 bp fragment of the viral genomic DNA containing the polyhedrin gene and other 12 genes was cloned and sequenced. Gene comparison and phylogenetic analysis showed that IrfaNPV is a member of the Group I NPVs. However, the genomic organization of IrfaNPV is highly distinct. In addition, electron microscopy analysis showed that IrfaNPV is a single nucleocapsid NPV (SNPV). An inoculation assay showed that IrfaNPV is semi-permissive in the Trichoplusia ni cell line Tn-5B1-4. Bioassays on lethal concentration (LC50) and lethal time (LT50) were conducted to test the susceptibility of I. fasciata larvae to the virus. |
