| Title |
Phylogeny of a Novel “Helicobacter heilmannii” Organism from a Japanese Patient with Chronic Gastritis Based on DNA Sequence Analysis of 16S rRNA and Urease Genes |
| Author |
Takehisa Matsumoto1,2, Masatomo Kawakubo2, Mayumi Shiohara1,2,3, Toshiko Kumagai2, Eiko Hidaka2, Kazuyoshi Yamauchi2, Kozue Oana4, Kenji Matsuzawa5, Hiroyoshi Ota4, and Yoshiyuki Kawakami4* |
| Address |
1Department of Laboratory Medicine, Shinshu University Graduate School of Medicine, Asahi, Matsumoto 390-8621, Japan, 2Department of Laboratory Medicine, Shinshu University Hospital, Asahi, Matsumoto 390-8621, Japan, 3Department of Nursing, Shinshu University Hospital, Asahi, Matsumoto 390-8621, Japan, 4Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, Asahi, Matsumoto 390-8621, Japan, 5Maruko General Hospital, Maruko, Ueda 386-0493, Japan |
| Bibliography |
Journal of Microbiology, 47(2),201-207, 2009,
|
| DOI |
|
| Key Words |
“Helicobacter heilmannii”, HHLO, phylogeny, phylogenetic tree, 16S rRNA, urease gene |
| Abstract |
“Helicobacter heilmannii” is an uncultivable spiral-shaped bacterium inhabiting the human gastric mucosa. It is larger and more tightly-coiled than H. pylori. We encountered a patient with chronic gastritis infected a “H. heilmannii”-like organism (HHLO), designated as SH6. Gastric mucosa derived from the patient was orally ingested by specific pathogen free mice. Colonization of the mice by SH6 was confirmed by electron microscopy of gastric tissue specimens. In an attempt to characterize SH6, 16S rRNA and urease genes were sequenced. The 16S rRNA gene sequence was most similar (99.4%; 1,437/1,445 bp) to HHLO C4E from a cheetah. However, the urease gene sequence displayed low similarity (81.7%; 1,240/1,516 bp) with HHLO C4E. Taxonomic analysis disclosed that SH6 represents a novel strain and should constitute a novel taxon in the phylogenetic trees, being discriminated from any other taxon, with the ability of infecting human gastric mucosa. |
