| Title |
Intracellular Substrates of a Heme-Containing Ascorbate Oxidase in Pleurotus ostreatus |
| Author |
Seung-Rock Lee1*, Woo-Jeong Joo2, Yong-Un Baek2, Youn-Kyong Lee2, Seong-Woon Yu2, Yeon-Ran Kim2, Kee-Oh Chay1, Seung-Hyun Cho2, and Sa-Ouk Kang2* |
| Address |
1Department of Biochemistry, Chonnam National University Medical School, Gwangju 501-190, Republic of Korea, 2Laboratory of Biophysics, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea |
| Bibliography |
Journal of Microbiology, 47(2),178-186, 2009,
|
| DOI |
|
| Key Words |
ascorbate oxidase, substrates, Pleurotus ostreatus |
| Abstract |
A novel heme-containing ascorbate oxidase isolated from oyster mushroom, Pleurotus ostreatus, catalyzes oxidation of ascorbic acid (Kim et al., 1996). In this report, we describe the identification of intracellular substrates of the enzyme in the mushroom. Six compounds, which can serve as substrate of the heme-containing ascorbate oxidase, were identified as L-ascorbic acid, D-erythroascorbic acid, 5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, 5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid, 5-methyl-5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, and 5-methyl-5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid. All of the compounds were oxidized at a significant rate by the heme-containing ascorbate oxidase. Oxidation of the compounds produced equimolar amounts of hydrogen peroxide per mole of substrate. |
