| Title |
Genetic Diversity of Chromosomal Metallo-β-Lactamase Genes in Clinical Isolates of Elizabethkingia meningoseptica from Korea |
| Author |
Jong Hwa Yum1, Eun Young Lee2, Sung-Ho Hur3, Seok Hoon Jeong2*, Hyukmin Lee4, Dongeun Yong2, Yunsop Chong2, Eun-Woo Lee5, Patrice Nordmann6, and Kyungwon Lee2 |
| Address |
1Department of Clinical Laboratory Science, Dong-eui University, Busan 614-714, Republic of Korea, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-749, Republic of Korea, 3Department of Clinical Laboratory Science, Dong-eui Institute of Technology University, Busan 614-715, Republic of Korea, 4Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang 412-270, Republic of Korea, 5Department of Life Science and Biotechnology, Dong-eui University, Busan 614-714, Republic of Korea, 6Service de Bacteriologie-Virologie, Hopital de Bicetre, Le Kremlin-Bicetre Cedex, France |
| Bibliography |
Journal of Microbiology, 48(3),358-364, 2010,
|
| DOI |
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| Key Words |
E. meningoseptica, metallo-β-lactamase, GOB, BlaB, cluster |
| Abstract |
This study was performed to characterize the chromosomal metallo-β-lactamases (MBLs) of Elizabethkingia meningoseptica isolated from Korea and to propose a clustering method of BlaB and GOB MBLs based on their amino acid similarities. Chromosomal MBL genes were amplified by PCR from 31 clinical isolates of E.
meningoseptica. These PCR products were then cloned into a vector and electrotransformed into E. coli DH5α. Nucleotide sequencing was performed by the dideoxy chain termination method using PCR products or cloned DNA fragments. Antimicrobial susceptibilities were determined by the agar dilution method. PCR
experiments showed that all 31 E. meningoseptica isolates contained both the blaB and the blaGOB genes. DNA sequence analysis revealed that E. meningoseptica isolates possessed seven types of blaB gene, including five novel variants (blaB-9 to blaB-13) and 11 types of blaGOB gene, including 10 novel variants (blaGOB-8 to blaGOB-17). The most common combination of MBL was BlaB-12 plus GOB-17 (n=19). Minimum inhibitory concentrations of imipenem and meropenem for the electrotransformants harboring novel BlaB and GOB MBLs were two- or four-fold higher than those for the recipient E. coli DH5α. BlaB and GOB MBLs were
grouped in three and six clusters including fifteen novel variants, respectively, based on amino acid similarities. |
