| Title |
Oceanobacillus manasiensis sp. nov., a Moderately Halophilic Bacterium Isolated from the Salt Lakes of Xinjiang, China |
| Author |
Lei Wang1,2, Wen-Yan Liu2,3, Zhi-Jing Gu2, San-Feng Chen1,2, and Su-Sheng Yang3* |
| Address |
1State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P. R. China, 2Key Laboratory for Agro-Microbial Resource and Application, Ministry of Agriculture, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing100193, P. R. China, 3National Engineering Laboratory of Biohydrometallurgy, General Research Institute for Nonferrous Metals, Beijing100088, P. R. China |
| Bibliography |
Journal of Microbiology, 48(3),312-317, 2010,
|
| DOI |
|
| Key Words |
Oceanobacillus manasiensis sp. nov., moderate halophile, salt lake, Gram-positive |
| Abstract |
Three Gram reaction positive, rod-shaped, moderately motile halophilic bacterial strains, designated YD3-56T, YD16, and YH29, were isolated from the sediments of Manasi and Aiding salt lakes in the Xinjiang region of China, respectively. The strains grew optimally at 30-37°C, pH 8-11, in the presence of 5-10% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains were closely related to members of the genus Oceanobacillus, exhibiting 99.1-99.2% similarity to O. kapialis KCTC 13177T, 99.2-99.3% to O. picturae KCTC 3821T, and 94.2-96% sequence similarity to other described Oceanobacillus species. SDS-PAGE of whole cell proteins preparations demonstrated that the strains exhibited high similarity to each other, but distinguished from O. kapialis KCTC 13177T and O. picturae KCTC 3821T (75%). DNA-DNA hybridization revealed that the similarity between the representative strain YD3-56T and O. kapialis KCTC 13177T was 35.3%, and the similarity between YD3-56T and O. picturae KCTC 3821T was 22.3%. Chemotaxonomic analysis of the strains showed menaquinone-7 was the predominant respiratory quinine. Major cellular fatty acids were anteiso-C15:0 and anteiso-C17:0. The polar lipid pattern for strain YD3-56T predominantly contained phosphatidylcholine, and trace to moderate amounts of phosphatidyl ethanolamine
and hydroxy-phosphatidyl ethanolamine. The diamino acid in murein was meso-diaminopimelic acid. The DNA G+C content of the strains was 39.7-40.1 mol%. On the basis of these results, the three strains should be classified as a novel species of the genus Oceanobacillus, for which the name Oceanobacillus manasiensis sp.
nov. has been proposed, with the type strain as YD3-56T (=CGMCC 1.9105T =NBRC 105903T). |
