| Title |
High Efficiency Transformation by Electroporation of Yarrowia lipolytica |
| Author |
Jia-Hung Wang, Wenpin Hung, and Shu-Hsien Tsai* |
| Address |
Material and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu 300, Taiwan |
| Bibliography |
Journal of Microbiology, 49(3),469-472, 2011,
|
| DOI |
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| Key Words |
dithiothreitol, electroporation, lithium acetate, transformation, Yarrowia lipolytica |
| Abstract |
Yarrowia lipolytica was usually transformed by heat shock, but linearized integrative vectors always resulted in a low transformation efficiency when electroporation was used. To develop a high efficiency integrative transformation method by electroporation of Y. lipolytica, we report here that pretreatment of Y. lipolytica with 150 mM LiAc for 1 h before electroporation will approximately 30-fold of increase transformation efficiency. A cell concentration of 1010/ml and instrument settings of 1.5 kV will generate the highest transformation efficiencies. We have developed a procedure to transform Y. lipolytica that will be able to yield an efficiency of 2.1×104 transformants/μg for integrative linear DNA. With our modifications, the electroporation procedures became a very efficient and reliable tool for Y. lipolytica transformation. |
