Title Shewanella upenei sp. nov., a Lipolytic Bacterium Isolated from Bensasi Goatfish Upeneus bensasi
Author Kyung-Kil Kim1, Young-Ok Kim1, Sooyeon Park2, So-Jung Kang2, Bo-Hye Nam1, Doo Nam Kim3, Tae-Kwang Oh2, and Jung-Hoon Yoon2*
Address 1Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Busan 619-705, Republic of Korea, 2Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea, 3Fisheries Resources Research Division, National Fisheries Research and Development Institute (NFRDI), Busan 619-705, Republic of Korea
Bibliography Journal of Microbiology, 49(3),381-386, 2011,
Key Words Shewanella upenei, taxonomy, new species
Abstract A Gram-staining-negative, motile, non-spore-forming and rod-shaped bacterial strain, 20-23RT, was isolated from intestine of bensasi goatfish, Upeneus bensasi, and its taxonomic position was investigated by using a polyphasic study. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 20-23RT belonged to the genus Shewanella. Strain 20-23RT exhibited 16S rRNA gene sequence similarity values of 99.5, 99.2, and 97.5% to Shewanella algae ATCC 51192T, Shewanella haliotis DW01T, and Shewanella chilikensis JC5T, respectively. Strain 20-23RT exhibited 93.1-96.0% 16S rRNA gene sequence similarity to the other Shewanella species. It also exhibited 98.3-98.4% gyrB sequence similarity to the type strains of S. algae and S. haliotis. Strain 20-23RT contained simultaneously both menaquinones and ubiquinones; the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-8 and Q-7. The fatty acid profiles of strain 20-23RT, S. algae KCTC 22552T and S. haliotis KCTC 12896T were similar; major components were iso-C15:0, C16:0, C16:1 ω7c and/or iso-C15:0 2-OH and C17:1 ω8c. The DNA G+C content of strain 20-23RT was 53.9 mol%. Differential phenotypic properties and genetic distinctiveness of strain 20-23RT, together with the phylogenetic distinctiveness, revealed that this strain is distinguishable from recognized Shewanella species. On the basis of the data presented, strain 20-23RT represents a novel species of the genus Shewanella, for which the name Shewanella upenei sp. nov. is proposed. The type strain is 20-23RT (=KCTC 22806T =CCUG 58400T).