Title NOTE] Complete Sequence and Organization of the Sphingobium chungbukense DJ77 pSY2 Plasmid
Author Sun-Mi Yeon and Young-Chang Kim*
Address Department of Microbiology, Chungbuk National University, Chungbuk 361-763, Republic of Korea
Bibliography Journal of Microbiology, 49(4),684-688, 2011,
DOI
Key Words plasmid, pSY2, characterization, Sphingobium chungbukense DJ77
Abstract Sphingobium chungbukense DJ77 is capable of metabolizing priority chemicals of human health concern such as polycyclic aromatic hydrocarbons (PAHs), extracellular polysaccharide (EPS), and antibiotics. Here, we report the complete DNA and genetic organization of the plasmid pSY2 from strain DJ77. A DNA sequence analysis revealed that pSY2 comprises 18,779 bp encoding 22 open reading frames (ORFs) with 59.5% G+C content. The ORFs on pSY2 were classified into DNA replication, conjugative function, transposition, plasmid stability/partition, and other functional groups (transport, fatty acid biosynthesis, stress, and growth rate regulation). Three ORFs on pSY2 were hypothetical proteins.