| Title |
NOTE] A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi |
| Author |
Yuanyuan Chen1, Bernard A. Prior2, Guiyang Shi1, and Zhengxiang Wang1* |
| Address |
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, P. R. China, 2Department of Microbiology, School of Biological Sciences, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa |
| Bibliography |
Journal of Microbiology, 49(4),675-679, 2011,
|
| DOI |
|
| Key Words |
filamentous fungi, genomic DNA extraction, alkaline lysis, internal transcribed spacer regions, identification |
| Abstract |
In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal
with a large number of filamentous fungal isolates in the same batch, was established. The filamentous
fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR
amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted
DNA also can be used to amplify other protein-coding genes for fungal identification. This method
can be used for rapid systematic identification of filamentous fungal isolates. |
