| Title |
Cyclic Lipopeptide Profile of Three Bacillus subtilis Strains; Antagonists of Fusarium Head Blight |
| Author |
Christopher A. Dunlap1*, David A. Schisler1, Neil P. Price2, and Steven F. Vaughn3 |
| Address |
1Crop Bioprotection, Research Units, National Center for Agricultural Utilization Research, Agricultural Research Service, USA, 2Bioproduct and Biocatalysis, Research Units, National Center for Agricultural Utilization Research, Agricultural Research Service, USA, 3New Crops and Processing, Research Units, National Center for Agricultural Utilization Research, Agricultural Research Service, USA |
| Bibliography |
Journal of Microbiology, 49(4),603-609, 2011,
|
| DOI |
|
| Key Words |
Bacillus, iturin, fengycin, wheat, biocontrol, lipopeptides |
| Abstract |
The objective of the study was to identify the lipopetides associated with three Bacillus subtilis strains.
The strains are antagonists of Gibberella zeae, and have been shown to be effective in reducing Fusarium
head blight in wheat. The lipopeptide profile of three B. subtilis strains (AS43.3, AS43.4, and OH131.1)
was determined using mass spectroscopy. Strains AS43.3 and AS43.4 produced the anti-fungal lipopeptides
from the iturin and fengycin family during the stationary growth phase. All three strains produced the
lipopeptide surfactin at different growth times. Strain OH131.1 only produced surfactin under these conditions.
The antifungal activity of the culture supernatant and individual lipopeptides was determined by the inhibition
of G. zeae. Cell-free supernatant from strains AS43.3 and AS43.4 demonstrated strong antibiosis of G. zeae,
while strain OH131.1 had no antibiosis activity. These results suggest a different mechanism of antagonism
for strain OH131.1, relative to AS43.3 and AS43.4. |
