| Title |
Characterization of Antibiotic Resistance Determinants in Oral Biofilms |
| Author |
Seon-Mi Kim1, Hyeong C. Kim2, and Seok-Woo S. Lee3,4* |
| Address |
1Department of Pediatric Dentistry, School of Dentistry, Dental Science Research Institute and 2nd stage of BK21, Chonnam National University, Gwangju 500-757, Republic of Korea, 2Division of Periodontics, College of Dental Medicine, Columbia University, New York, NY, USA, 3Division of Periodontics, School of Dentistry, Marquette University, Milwaukee, WI, USA, 4Departments of Dental Education and Periodontics, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 500-757, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(4),595-602, 2011,
|
| DOI |
|
| Key Words |
antibiotic resistance, resistance determinants, periodontitis, oral biofilm, polymerase chain reaction |
| Abstract |
Oral biofilms contain numerous antibiotic resistance determinants that can be transferred within or outside
of the oral cavity. The aim of this study was to evaluate the prevalence and the relative level of antibiotic
resistance determinants from oral biofilms. Oral biofilm samples that were collected from healthy subjects
and periodontitis patients were subjected to qualitative and quantitative analyses for selected antibiotic resistance
determinants using PCR. The prevalence of tet(Q), tet(M), cfxA, and blaTEM was very high both in
the patient and the healthy subject group, with a tendency toward higher values in the patient group,
with the exception of erm(F), which was more prevalent in the healthy group. The two extended spectrum
β-lactam (ESBL) resistance determinants blaSHV and blaTEM showed a dramatic difference, as blaTEM was
present in all of the samples and blaSHV was not found at all. The aacA-aphD, vanA, and mecA genes were
rarely detected, suggesting that they are not common in oral bacteria. A quantitative PCR analysis showed
that the relative amount of resistance determinants present in oral biofilms of the patient group was much
greater than that of the healthy group, exhibiting 17-, 13-, 145-, and 3-fold increases for tet(Q), tet(M),
erm(F), and cfxA, respectively. The results of this study suggest that the oral antibiotic resistome is more
diverse and abundant in periodontitis patients than in healthy subjects, suggesting that there is a difference
in the diversity and distribution of antibiotic resistance in oral biofilms associated with health and disease. |
