| Title |
NOTE] Leucobacter denitrificans sp. nov., Isolated from Cow Dung |
| Author |
Hang-Yeon Weon1, Rangasamy Anandham2, Tomohiko Tamura3, Moriyuki Hamada3, Soo-Jin Kim1, Yi-Seul Kim1, Ken-ichiro Suzuki3, and Soon-Wo Kwon1* |
| Address |
1Korean Agricultural Culture Collection (KACC), National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea , 2Department of Agricultural Microbiology, Agricultural College and Research Institute, Madurai, 625-105, India, 3NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, Kisarazu, Chiba 292-0818, Japan |
| Bibliography |
Journal of Microbiology, 50(1),161-165, 2012,
|
| DOI |
|
| Key Words |
Leucobacter denitrificans sp. nov., taxonomy, 16S rRNA gene, phylogeny, new species |
| Abstract |
The bacterial strain M1T8B10T was isolated from cow dung
in Suwon, Republic of Korea. The strain was a Gram stainpositive
rod, nonmotile, and non-spore-forming. According
to 16S rRNA gene sequence analysis, the strain fell within
the clade of the genus Leucobacter, showing the highest sequence
similarities with Leucobacter aridicollis L-9T (98.7%),
Leucobacter iarius 40T (98.4%), and Leucobacter komagatae
JCM 9414T (98.2%). Cell-wall peptidoglycan contained the
diagnostic diamino acid 2,4-diaminobutyric acid of the genus
Leucobacter, showing B-type cross-linked peptidoglycans.
The major fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-
C17:0. The quinone system consisted of the menaquinones
MK-11 (78%) and MK-10 (22%). The polar lipid profiles
contained diphosphatidylglycerol, phosphatidylglycerol, and
an unidentified glycolipid. Differences in several physiological
features including nitrate reduction enabled the isolate
to be differentiated from all recognized Leucobacter
species. Based on these phylogenetic, chemotaxonomic, and
phenotypic results, the isolate represents a novel species,
for which the name Leucobacter denitrificans sp. nov. is
proposed. The type strain is M1T8B10T (=KACC 14055T
=NBRC 106309T). |
