| Title |
Acinetobacter baumannii Outer Membrane Protein A Modulates the Biogenesis of Outer Membrane Vesicles |
| Author |
Dong Chan Moon1, Chul Hee Choi1, Jung Hwa Lee1, Chi-Won Choi2, Hye-Yeon Kim3, Jeong Soon Park3, Seung Il Kim2*, and Je Chul Lee1* |
| Address |
1Department of Microbiology, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea, 2Division of Life Science, Korea Basic Science Institute, Daejeon 305-806, Republic of Korea, 3Division of Magnetic Resonance Research, Korea Basic Science Institute, Chungbuk 363-883, Republic of Korea |
| Bibliography |
Journal of Microbiology, 50(1),155-160, 2012,
|
| DOI |
|
| Key Words |
outer membrane protein A, lipopolysaccharides, peptidoglycan, protein sorting |
| Abstract |
Acinetobacter baumannii secretes outer membrane vesicles
(OMVs) during both in vitro and in vivo growth, but the
biogenesis mechanism by which A. baumannii produces
OMVs remains undefined. Outer membrane protein A of
A. baumannii (AbOmpA) is a major protein in the outer
membrane and the C-terminus of AbOmpA interacts with
diaminopimelate of peptidoglycan. This study investigated
the role of AbOmpA in the biogenesis of A. baumannii
OMVs. Quantitative and qualitative approaches were used
to analyze OMV biogenesis in A. baumannii ATCC 19606T
and an isogenic ΔAbOmpA mutant. OMV production was
significantly increased in the ΔAbOmpA mutant compared
to wild-type bacteria as demonstrated by quantitation of
proteins and lipopolysaccharides (LPS) packaged in OMVs.
LPS profiles prepared from OMVs from wild-type bacteria
and the ΔAbOmpA mutant had identical patterns, but
proteomic analysis showed different protein constituents in
OMVs from wild-type bacteria compared to the ΔAbOmpA
mutant. In conclusion, AbOmpA influences OMV biogenesis
by controlling OMV production and protein composition. |
