| Title |
Heat- and Cold-Shock Responses in Fusarium graminearum 3 Acetyl- and 15 Acetyl-Deoxynivalenol Chemotypes |
| Author |
Vladimir Vujanovic*, Yit Kheng Goh, and Prasad Daida |
| Address |
Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada |
| Bibliography |
Journal of Microbiology, 50(1),97-102, 2012,
|
| DOI |
|
| Key Words |
Fusarium graminearum, chemotypes, heat-shock, cold-shock, INA activity, mycotoxins |
| Abstract |
Fusarium graminearum Schwabe is the primary cause of
Fusarium head blight (FHB) in North America. Chemically
distinct F. graminearum sub-populations can be identified
based on the type or composition of deoxynivalenol (DON)
mycotoxin derivatives, including 3-acetyl (3-ADON) and
15-acetyl (15-ADON). The evaluation of randomly selected
3-ADON and 15-ADON isolates, collected from spring
wheat throughout Canada, was performed using thin layer
chromatography (TLC), high-performance liquid chromatography
(HPLC), ice-nucleation activity (INA), and heat and
cold tolerance tests conducted within a temperature range
of -70°C to 65°C. The results indicated that the 3-ADON
sub-population, which is responsible for the highest disease
severity and has rapidly displaced the 15-ADON sub-population,
produces more DON and zearalenone (ZEA) than
the 15-ADON sub-population when exposed to heat and
cold. Following exposures (1 and 2 h) to extremely high or
low temperatures, 3-ADON isolates exhibited faster mycelial
growth than 15-ADON isolates. In addition, the warmest
temperature at which INA activity occurred was in 3-ADON
(-3.6°C) vs. 15-ADON (-5.1°C). Taken together, these features
suggest that the newly emerging 3-ADON sub-population
is more resilient than the resident 15-ADON sub-population.
Overall, the differences between the two sub-populations
could provide new insights into FHB epidemiology and if
validated under field conditions, may provide important
information for predicting future FHB epidemics. |
