| Title |
Effects of a Dark-Septate Endophytic Isolate LBF-2 on the Medicinal Plant Lycium barbarum L. |
| Author |
Hai-han Zhang1, Ming Tang2*, Hui Chen2, and Ya-jun Wang3 |
| Address |
1College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China, 2College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China, 3Ningxia Wolfberry Engineering and Technology Research Center, Yinchuan, Ningxia 750002, P. R. China |
| Bibliography |
Journal of Microbiology, 50(1),91-96, 2012,
|
| DOI |
|
| Key Words |
chlorophyll fluorescence, dark septate endophyte, Lycium barbarum, medicinal plant |
| Abstract |
Dark septate endophytes (DSE) are ubiquitous root associated
fungi; however, our understanding of their ecological
function remains unclear. Here, we investigated the positive
effect of a DSE fungus on its host plant Lycium barbarum
L. A DSE isolate, LBF-2, isolated from the roots of L. barbarum,
was inoculated onto the roots of plants, which were
grown under greenhouse conditions for five weeks. The result
of molecular analyses of internal transcribed spacer regions
indicated that LBF-2 was 96% similar to Paraphoma chrysanthemicola.
Melanized septate hyphae were observed in
the root cortical cells of L. barbarum using a light microscope.
Inoculation with LBF-2 increased the total biomass by 39.2%
and also enhanced chlorophyll fluorescence. Inoculation
increased the concentration of total chlorophyll by 22.8%
and of chlorophyll a by 21.3%, relative to uninoculated
controls. These data indicate that the LBF-2 isolate might
be used to facilitate the cultivation of L. barbarum, which
has medicinal applications. |
