Title |
Phenotypic and Phylogenetic Analysis of Lactic Acid Bacteria Isolated from Forage Crops and Grasses in the Tibetan Plateau |
Author |
Huili Pang1, Zhongfang Tan1, Guangyong Qin1, Yanping Wang1, Zongwei Li1, Qingsheng Jin2, and Yimin Cai3* |
Address |
1Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, 450052 Zhengzhou, Henan, P. R. China, 2Institute of Crops and Utilization of Nuclear Technology, Zhejiang Academy of Agricultural Sciences, 310021 Hangzhou, Zhejiang, P. R. China, 3Japan International Research Center for Agricultural Sciences, 305-8686 Tsukuba, Ibaraki, Japan |
Bibliography |
Journal of Microbiology, 50(1),63-71, 2012,
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DOI |
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Key Words |
forage, epiphytic microflora, lactic acid bacteria, 16S rDNA, recA gene |
Abstract |
A total of 140 lactic acid bacteria (LAB) strains were isolated
from corn, alfalfa, clover, sainfoin, and Indian goosegrass
in the Tibetan Plateau. According to phenotypic and chemotaxonomic
characteristics, 16S rDNA sequence, and recA
gene PCR amplification, these LAB isolates were identified
as belonging to five genera and nine species. Corn contained
more LAB species than other forage crops. Leuconostoc
pseudomesenteroides, Lactococcus lactis subsp. lactis, Lactobacillus
brevis, and Weissella paramesenteroides were dominant
members of the LAB population on alfalfa, clover, sainfoin,
and Indian goosegrass, respectively. The comprehensive
16S rDNA and recA-based approach effectively described
the LAB community structure of the relatively abundant LAB
species distributed on different forage crops. This is the
first report describing the diversity and natural populations
of LAB associated with Tibetan forage crops, and most isolates
grow well at or below 10°C. The results will be valuable for
the future design of appropriate inoculants for silage fermentation
in this very cold area. |