| Title |
Microflora Profiling of Infected Root Canal before and after Treatment Using Culture-Independent Methods |
| Author |
Yasuhiro Ito1,2, Takuichi Sato2*, Keiko Yamaki1, Gen Mayanagi1, Kazuhiro Hashimoto1, Hidetoshi Shimauchi1, and Nobuhiro Takahashi2 |
| Address |
1Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan, 2Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan |
| Bibliography |
Journal of Microbiology, 50(1),58-62, 2012,
|
| DOI |
|
| Key Words |
16S ribosomal RNA, microflora, PCR, root canals |
| Abstract |
This study aimed to profile the microflora in infected root
canals before and after root canal treatment using cultureindependent
methods. Six infected root canals in singlerooted
teeth with periapical lesions from five subjects were
included. Quantification of total bacteria was performed by
real-time PCR with primers targeting 16S rRNA genes.
PCR products with universal 16S rRNA gene primers were
cloned and partially sequenced, and bacterial identification
at the species level was performed by comparative analysis
with the GenBank database. The concentration of extracted
DNA before treatment was higher than that after root canal
treatment, although the difference was not statistically
significant. Sequence analysis revealed that oral bacteria
such as Fusobacterium, Streptococcus, Olsenella, and Pseudoramibacter
detected in cases before root canal treatment
disappeared after treatment. These results suggest that the
root canal microflora are distinct before and after root
canal treatment, and that treatment changes the microflora
in both quantity and quality. |
