| Title |
Growth Promotion of Xanthium italicum by Application of Rhizobacterial Isolates of Bacillus aryabhattai in Microcosm Soil |
| Author |
Sol Lee1, Jong-Ok Ka2, and Hong-Gyu Song1* |
| Address |
1Department of Biological Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea |
| Bibliography |
Journal of Microbiology, 50(1),45-49, 2012,
|
| DOI |
|
| Key Words |
plant growth promotion, rhizobacteria, Xanthium italicum, Bacillus aryabhattai |
| Abstract |
This study was conducted using rhizobacteria, which are
able to exert beneficial effects upon plant growth in the infertile
soil collected from barren lakeside areas. Four strains
of plant growth promoting bacteria were isolated from the
rhizosphere of a common wild plant, Erigeron canadensis.
Isolated strains LS9, LS11, LS12, and LS15 were identified
as Bacillus aryabhattai by 16S rDNA sequence analysis. B.
aryabhattai LS9, LS11, LS12, and LS15 could solubilize
577.9, 676.8, 623.6, and 581.3 mg/L of 0.5% insoluble calcium
phosphate within 2 days of incubation. Production of indole
acetic acid, a typical growth promoting phytohormone
auxin, by strain LS15 was 471.3 mg/L in 2 days with the addition
of auxin precursor L-tryptophan. All the strains also
produced other phytohormones such as indole butyric acid,
gibberellins, and abscisic acid, and strain LS15 showed the
highest production rate of gibberellin (GA3), 119.0 μg/mg
protein. Isolated bacteria were used in a microcosm test for
growth of wild plant Xanthium italicum, which can be utilized
as a pioneer plant in barren lands. Seed germination
was facilitated, and the lengths of roots, and shoots and the
dry weights of germinated seedlings after 16 days were
higher than those of the uninoculated control plants. Root
lengths of seedlings of X. italicum increased by 121.1% in
LS11-treated samples after 16 days. This plant growth-promoting
capability of B. aryabhattai strains may be utilized
as an environmentally friendly means of revegetating barren
lands, especially sensitive areas such as lakeside lands. |
