Title NOTE] Identification of Chaperones in Freeze Tolerance in Saccharomyces cerevisiae
Author Mahendran Chinnamara Naicker, I Seul Jo, and Hana Im*
Address Department of Molecular Biology, Sejong University, Seoul 143-747, Republic of Korea
Bibliography Journal of Microbiology, 50(5),882-887, 2012,
Key Words chaperone, yeast, freeze tolerance, Saccharomyces cerevisiae, cold shock response
Abstract Exposure to low temperatures reduces protein folding rates and induces the cold denaturation of proteins. Considering the roles played by chaperones in facilitating protein folding and preventing protein aggregation, chaperones must exist that confer tolerance to cold stress. Here, yeast strains lacking individual chaperones were screened for reduced freezing tolerance. In total, 19 of 82 chaperone-deleted strains tested were more sensitive to freeze-thaw treatment than wild-type cells. The reintroduction of the respective chaperone genes into the deletion mutants recovered the freeze tolerance. The freeze sensitivity of the chaperone-knockout strains was also retained in the presence of 20% glycerol.