Title NOTE] Regulatory Characteristics of the Vibrio vulnificus rtxHCA Operon Encoding a MARTX Toxin
Author Jinseo Park, Seung Min Kim, Hee Gon Jeong, and Sang Ho Choi*
Address National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 151-921, Republic of Korea
Bibliography Journal of Microbiology, 50(5),878-881, 2012,
Key Words V. vulnificus rtxA, MARTX
Abstract Vibrio vulnificus MARTX encoded by rtxA, an open reading frame of the rtxHCA operon, is essential for virulence in vitro and in mice. In this study, a primer extension analysis revealed that transcription of the rtxHCA operon begins at a single site, and is under the direction of a single promoter, PrtxHCA. PrtxHCA activity appeared at the beginning of growth and reached a maximum in mid-exponential phase. PrtxHCA activity was induced by exposure to INT-407 cells, and the membrane fraction of INT-407 cells was the most effective for the induction.