Title NOTE] Construction and Characterisation of an Antifungal Recombinant Bacillus thuringiensis with an Expanded Host Spectrum
Author Qin Liu1, Jong Yul Roh2, Yong Wang3, Jae Young Choi1, Xue Ying Tao1, Jae Su Kim4, and Yeon Ho Je1,5*
Address 1Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Republic of Korea, 2Division of Medical Entomology, Korea National Institute of Health, Chungbuk 363-951, Republic of Korea, 3School of Life Science, Lanzhou University, 99 Dong Ganxi Road, Lanzhou 730000, P. R. China, 4Department of Agricultural Biology, College of Agricultural Life Science, Chonbuk National University, Jeonju 561-756, Republic of Korea, 5Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea
Bibliography Journal of Microbiology, 50(5),874-877, 2012,
Key Words Bacillus thuringiensis, antifungal activity, cry1E, insecticidal activity
Abstract A novel antifungal Bacillus thuringiensis strain 19–22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests.