Title NOTE] Next-Generation Sequencing-Based Genome-Wide Mutation Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain
Author Chang-Soo Lee1, Jae-Young Nam1, Eun-Suk Son1, O-chul Kwon1, Woorijarang Han1, Jae-Yong Cho2, and Young-Jin Park1*
Address 1Department of Applied Biochemistry, Konkuk University, Chung-Ju 380-701, Republic of Korea, 2Division of Animal Science and Biotechnology, Sangji University, Wonju 220-702, Republic of Korea
Bibliography Journal of Microbiology, 50(5),860-863, 2012,
Key Words mutation, SNP, InDel, genome, Corynebacterium glutamicum
Abstract In order to identify single nucleotide polymorphism and insertion/deletion mutations, we performed whole-genome re-sequencing of the enhanced L-lysine-producing Corynebacterium glutamicum ATCC 21300 strain. In total, 142 single nucleotide polymorphisms and 477 insertion/deletion mutations were identified in the ATCC 21300 strain when compared to 3,434 predicted genes of the wild-type C. glutamicum ATCC 13032 strain. Among them, 110 transitions and 29 transversions of single nucleotide polymorphisms were found from genes of the ATCC 21300 strain. In addition, 11 genes, involved in the L-lysine biosynthetic pathway and central carbohydrate metabolism, contained mutations including single nucleotide polymorphisms and insertions/deletions. Interestingly, RT-PCR analysis of these 11 genes indicated that they were normally expressed in the ATCC 21300 strain. This information of genome-wide gene-associated variations will be useful for genome breeding of C. glutamicum in order to develop an industrial amino acidproducing strain with minimal mutation.