| Title |
NOTE] Burkholderia denitrificans sp. nov., Isolated from the Soil of Dokdo Island, Korea |
| Author |
Chang-Muk Lee Lee1, Hang-Yeon Weon2, Sang-Hong Yoon1, Soo-Jin Kim2, Bon-Sung Koo1, and Soon-Wo Kwon2* |
| Address |
1Functional Biomaterial Division, Agricultural Microbiology Team, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea, 2Korean Agricultural Culture Collection (KACC), Agricultural Microbiology Team, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea |
| Bibliography |
Journal of Microbiology, 50(5),855-859, 2012,
|
| DOI |
|
| Key Words |
Burkholderia denitrificans, 16S rRNA gene sequence, taxonomy |
| Abstract |
A novel, Gram-negative, bacterial strain KIS30-44T was identified from wet forest soil collected on the Korean island of Dokdo. Growth of the strain was observed at 15–30°C, pH 5−9, 0–3% NaCl, and 950 mM KNO3. KIS30-44T reduced nitrate to nitrogen gas. Analysis of the 16S rRNA gene sequence showed that KIS30-44T was phylogenetically related to Burkholderia sacchari, Burkholderia mimosarum, and Burkholderia oxyphila (98.1%, 98.0%, and 98.0% sequence similarity, respectively). The genomic G+C content was 63.5 mol%. KIS30-44T exhibited less than 52% DNA-DNA relatedness with the type strains of 9 closely related Burkholderia species. The major isoprenoid quinone was Q-8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminolipids. The major fatty acids in KIS30-44T were C16:0, C18:1 ω7c and summed feature 3 (iso-C15:0 2-OH and C16:1 ω7c), and the strain contained half the amount of C17:0 cyclo found in the 9 closely related Burkholderia species. The results of these phenotypic, 16S rRNA gene sequence, DNA-DNA hybridization, and chemotaxonomic data indicate that KIS30-44T represents a novel species within the genus Burkholderia, for which the name Burkholderia denitrificans (Type strain KIS30-44T =KACC 12733T =DSM 24336T) is proposed. |
