| Title | NOTE] Comparative Assessment of the Intracellular Survival of the Burkholderia pseudomallei bopC Mutant |
|---|---|
| Author | Varintip Srinon1, Sunsiree Muangman1, Nithima Imyaem1, Veerachat Muangsombut1, Natalie R. Lazar Adler2, Edouard E. Galyov2, and Sunee Korbsrisate1* |
| Address | 1Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand, 2Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, United Kingdom |
| Bibliography | Journal of Microbiology, 51(4),522-526, 2013, |
| DOI | 10.1007/s12275-013-2557-3 |
| Key Words | Burkholderia pseudomallei, BopC, type III secretion effector, intracellular survival, phagocyte |
| Abstract | Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative saprophytic bacterium capable of surviving within phagocytic cells. To assess the role of BopC (a type III secreted effector protein) in the pathogenesis of B. pseudomallei, a B. pseudomallei bopC mutant was used to infect J774A.1 macrophage-like cells. The bopC mutant showed significantly reduced intracellular survival in infected macrophages compared to wild-type B. pseudomallei. In addition, the bopC mutant displayed delayed escape from endocytic vesicles compared with the wild-type strain. This indicates that BopC is important, and at least in part, needed for intracellular survival of B. pseudomallei. |