| Title | The N3 Subdomain in A Domain of Fibronectin-Binding Protein B Isotype I Is an Independent Risk Determinant Predictive for Biofilm Formation of Staphylococcus aureus Clinical Isolates |
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| Author | An Sung Kwon1, Dong Hoon Lim2, Hyo Jung Shin1, Geon Park3, Jong H. Reu1, Hyo Jin Park1, Jungmin Kim4*, and Yong Lim1* |
| Address | 1Department of Microbiology, Medical School, Chosun University, Gwangju 501-759, Republic of Korea, 2Department of Urology, Medical School, Chosun University, Gwangju 501-759, Republic of Korea, 3Department of Laboratory Medicine, Medical School, Chosun University, Gwangju 501-759, Republic of Korea , 4Department of Microbiology, Kyungpook National University, School of Medicine, Daegu 700-422, Republic of Korea |
| Bibliography | Journal of Microbiology, 51(4),499-505, 2013, |
| DOI | 10.1007/s12275-013-3319-y |
| Key Words | fibronectin-binding proteins, N3 subdomains of FnBPB, biofilm, S. aureus |
| Abstract | Fibronectin-binding proteins (FnBP), FnBPA and FnBPB, are purported to be involved in biofilm formation of Staphylococcus aureus. This study was performed to find which of three consecutive N subdomains of the A domain in the FnBP is the key domain in FnBP. A total of 465 clinical isolates of S. aureus were examined for the biofilm forming capacity and the presence of N subdomains of FnBP. In the biofilm-positive strains, N2 and N3 subdomains of FnBPA, and N1 and N3 subdomains of FnBPB were significantly more prevalent. Multivariate logistic regression analysis of 246 biofilm-positive and 123 biofilm-negative strains identified only the FnBPB-N3 subdomain as an independent risk determinant predictive for biofilm-positive strains of S. aureus (Odds ratio [OR], 13.174; P<0.001). We also attempted to delete each of the fnbA-N2 and -N3 and fnbB-N1 and -N3 from S. aureus strain 8325-4 and examined the biofilm forming capacity in the derivative mutants. In agreement with the results of the multivariate regression analysis, deletion of either the fnbA-N2 or -N3, or fnbB-N1 did not significantly diminish the capacity of strain 8325-4 to develop a biofilm, while deletion of the fnbB-N3 did. Therefore, it is suggested that the FnBPB-N3 subdomain of isotype I may be a key domain in FnBP which is responsible for the causing biofilm formation in S. aureus clinical isolates. |