Title Proteomic Comparison between Salmonella Typhimurium and Salmonella Typhi
Author Yue Wang1, Kuan-Yeh Huang2,3, and Yanan Huo4
Address 1Department of Gynecology and Obstetrics, Women’s Hospital School of Medicine Zhejiang University, Hangzhou, P. R. China, 2Graduate Institute of Biomedical Sciences, Chang Gung University College of Medicine, Taoyuan, Taiwan, 3Molecualr Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan, 4Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine Zhejiang University, Hangzhou, P. R. China
Bibliography Journal of Microbiology, 52(1),71–76, 2014,
DOI 10.1007/s12275-014-3204-3
Key Words SILAC, proteome, Salmonella, typhoid, biomarker
Abstract The genus Salmonella contains more than 2500 serovars. While most cause the self-limiting gastroenteritis, a few serovars can elicit typhoid fever, a severe systemic infection. S. enterica subsp. enterica serovar Typhimurium and S. Typhi are the representatives of the gastroenteritis and typhoid fever types of Salmonella. In this study, we adopted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) technology to quantitatively compare the proteomes of the two serovars. We found several proteins with serovar- specific expression, which could be developed as new biomarkers for clinical serotype diagnosis. We found that flagella and chemotaxis genes were down-regulated in S. Typhi in comparison with S. Typhimurium. We attributed this observation to the fact that the smooth cellular structure of S. Typhi may better fit its systemic lifestyle. Instead of known virulence factors that were located within Salmonella Pathogenecity Islands, a number of core genes, which were involved in metabolism and transport of carbohydrates and amino acids, showed differential expression between the two serovars. Further studies on the roles of these differentially- expressed genes in the pathogenesis should be undertaken.