Title |
Optimization of Water Absorbing Exopolysaccharide Production on Local Cheap Substrates by Bacillus Strain CMG1403 Using One Variable at a Time Approach |
Author |
Muhammadi 1* and Muhammad Afzal2 |
Address |
1Department of Engineering Management, College of Electrical and Mechanical Engineering, National University of Science and Technology, Islamabad, Pakistan, 2Department of Basic Sciences, College of Electrical and Mechanical Engineering, National University of Science and Technology, Islamabad, Pakistan |
Bibliography |
Journal of Microbiology, 52(1),44–52, 2014,
|
DOI |
10.1007/s12275-014-2622-6
|
Key Words |
optimization, exopolysaccharide, OVAT, local cheap substrates |
Abstract |
Optimum culture conditions, and carbon and nitrogen sources
for production of water absorbing exopolysaccharide by
Bacillus strain CMG1403 on local cheap substrates were determined
using one variable at a time approach. Carbon
source was found to be sole substrate for EPS biosynthesis
in the presence of yeast extract that supported the growth
only and hence, indirectly enhanced the EPS yield. Whereas,
urea only coupled with carbon source could enhance the EPS
production but no effect on growth. The maximum yield of
EPS was obtained when Bacillus strain CMG1403 was grown
statically in neutral minimal medium with 25% volumetric
aeration at 30°C for 10 days. Under these optimum conditions,
a maximum yield of 2.71±0.024, 3.82±0.005, 4.33±0.021,
4.73±0.021, 4.85±0.024, and 5.52±0.016 g/L culture medium
was obtained with 20 g (sugar) of sweet whey, glucose, fructose,
sucrose, cane molasses and sugar beet the most efficient
one respectively as carbon sources. Thus, the present
study showed that under optimum culture conditions, the
local cheap substrates could be superior and efficient alternatives
to synthetic carbon sources providing way for an
economical production of water absorbing EPS by indigenous
soil bacterium Bacillus strain CMG1403. |