Title |
Analysis of Bacterial Diversity in Sponges Collected from Chuuk and Kosrae Islands in Micronesia |
Author |
In-Hye Jeong1, Kyoung-Ho Kim2, Hyi-Seung Lee3, and Jin-Sook Park1* |
Address |
1Department of Biological Science and Biotechnology, Hannam University, Daejeon 305-811, Republic of Korea, 2Department of Microbiology, Pukyong National University, Busan 608-737, Republic of Korea, 3Marine Natural Product Chemistry Laboratory, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea |
Bibliography |
Journal of Microbiology, 52(1),20–26, 2014,
|
DOI |
10.1007/s12275-014-3619-x
|
Key Words |
bacterial diversity, Chuuk Lagoon, Kosrae Island, Micronesia, sponge, 454 pyrosequencing |
Abstract |
The bacteria resident in sponges collected from Chuuk Lagoon
and Kosrae Island of Micronesia were investigated using the
16S rRNA gene PCR-tagged pyrosequencing method. These
sponges were clustered into 5 groups based on their bacterial
composition. Diversity indexes and cumulative rank abundance
curves showed the different compositions of bacterial
communities in the various groups of sponges. Reads related
to the phylum Chloroflexi were observed predominantly
(9.7–68.2%) in 9 sponges of 3 groups and unobserved in the
other 2 groups. The Chloroflexi-containing group had similar
bacterial patterns at the phylum and lower taxonomic levels,
for example, significant proportions of Acidobacteria, Gemmatimonadetes,
SBR1093, and PAUC34f were observed in
most members of this group. The three groups in the Chloroflexi-
containing group, however, showed some minor
differences in the composition and diversity. The other two
groups contained high proportions of Proteobacteria (>87%)
or Bacteroidetes (>61%) and different composition and diversity
compared to the Chloroflexi-containing group and each
other. Four pairs of specimens with the same species showed
similar bacterial profiles, but, the bacteria in sponges were
highly specific at the individual level. |