Title |
Pb Tolerance and Bioaccumulation by the Mycelia of Flammulina velutipes in Artificial Enrichment Medium |
Author |
Changwei Zhu, Zhengpeng Li, Decai Li, and Yan Xin |
Address |
College of Life Science, Anhui Science and Technology University, Fengyang, Anhui, 233100, P. R. China |
Bibliography |
Journal of Microbiology, 52(1),8–12, 2014,
|
DOI |
10.1007/s12275-014-2560-3
|
Key Words |
bioaccumulation, heavy metal, Pb, mushroom, Flammulina velutipes |
Abstract |
Mushrooms have the ability to accumulate high concentrations
of heavy metals, which gives them potential for use as
bioremediators of environmental contamination. The Pb2+
tolerance and accumulation ability of living mycelia of Flammulina
velutipes were studied in this work. Mycelial growth
was inhibited when exposed to 1 mM Pb2+. The colony diameter
on solid medium decreased almost 10% compared
with the control. Growth decreased almost 50% when the
Pb2+ concentration increased to 4 mM in the medium, with
the colony diameter decreasing from 80 mm to 43.4 mm,
and dry biomass production in liquid cultures decreasing
from 9.23±0.55 to 4.27±0.28 g/L. Lead ions were efficiently
accumulated in the mycelia. The amount of Pb2+ in the mycelia
increased with increasing Pb2+ concentration in the
medium, with the maximum concentration up to 707±91.4
mg/kg dry weight. We also show evidence that a large amount
of the Pb2+ was adsorbed to the mycelial surface, which may
indicate that an exclusion mechanism is involved in Pb
tolerance. These results demonstrate that F. velutipes could
be useful as a remediator of heavy metal contamination because
of the characteristics of high tolerance to Pb2+ and efficient
accumulation of Pb2+ ions by the mycelia. |