| Title | Synthetic Lethal Screen of NAA20, a Catalytic Subunit Gene of NatB N-Terminal Acetylase in Saccharomyces cerevisiae |
|---|---|
| Author | Kang-Eun Lee, Jun-Young Ahn, Jeong-Mok Kim, and Cheol-Sang Hwang* |
| Address | Department of Life Sciences, Pohang University of Science and Technology, Gyeongbuk 790-784, Republic of Korea |
| Bibliography | Journal of Microbiology, 52(10),842-848, 2014, |
| DOI | 10.1007/s12275-014-3694-z |
| Key Words | N-terminal acetylation, synthetic lethal screen, NatB, Naa20, synthetic genetic array |
| Abstract | The Saccharomyces cerevisiae NatB N-terminal acetylase contains a catalytic subunit Naa20 and an auxiliary subunit Naa25. To elucidate the cellular functions of the NatB, we utilized the Synthetic Genetic Array to screen for genes that are essential for cell growth in the absence of NAA20. The genome-wide synthetic lethal screen of NAA20 identified genes encoding for serine/threonine protein kinase Vps15, 1,3-beta-glucanosyltransferase Gas5, and a catabolic repression regulator Mig3. The present study suggests that the catalytic activity of the NatB N-terminal aceytase is involved in vacuolar protein sorting and cell wall maintenance. |