Title |
NOTE] A Protective Role of Methionine-R-Sulfoxide Reductase against Cadmium in Schizosaccharomyces pombe |
Author |
Chang-Jin Lim1, Hannah Jo1, and Kyunghoon Kim2* |
Address |
1Department of Biochemistry, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2Department of Biological Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea |
Bibliography |
Journal of Microbiology, 52(11),976–981, 2014,
|
DOI |
10.1007/s12275-014-3512-7
|
Key Words |
cadmium, fission yeast, methionine-R-sulfoxide
reductase, msrB+, Schizosaccharomyces pombe |
Abstract |
The Schizosaccharomyces pombe cells harboring the methionine-
R-sulfoxide reductase (MsrB)-overexpressing recombinant
plasmid pFMetSO exhibited better growth than vector
control cells, when shifted into fresh medium containing
cadmium chloride (abbreviated as Cd). Although both groups
of cells contained enhanced reactive oxygen species (ROS)
and nitric oxide (NO) levels in the presence of Cd, ROS and
NO levels were significantly lower in the S. pombe cells harboring
pFMetSO than in vector control cells. Conversely, the
S. pombe cells harboring pFMetSO possessed higher total
glutathione (GSH) levels and a greater reduced/oxidized GSH
ratio than vector control cells under the same conditions. |